LOCUS       40924_49262        4866 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD056, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 4866)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4866)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    405..410
                     /label=ZraI
                     /note="ZraI"
     misc_feature    408..2581
                     /note="CVD-CYA; device for the GC-adaptor assembly: 
                     cyanobacterial replicons"
     misc_feature    408..428
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    429..434
                     /label=XbaI
                     /note="XbaI"
     CDS             complement(585..1892)
                     /codon_start=1
                     /product="repA-pFDA"
                     /label=repA-pFDA
                     /note="repA-pFDA; derived from pPL2; 7; Fremyella
                     diplosiphon PCC7601"
                     /protein_id="AII99654.1"
                     /translation="MKHTTFPHSNIIAQSTATEGENFVLQTRLHASILLLTPASKLWYL
                     CRALDLSGRGYVVLSPQNLELLQEKKSTIYRWLKDGKDLGLFRCYSWAGNTLKISLGSL
                     WRACKKARIKNWGTVANNIPLEEILKNNGRRTVATAMTIQDWQERSRYAAKNQLNPLER
                     KCFEIPATADVLTPQTSPKLTSGGTTGVLHVGEKRIFVGRSFIPFGVSQKRISNELNSQ
                     PASCGVSVRTVQRHVERLEVKHRQLMQARREYREIAHRIKQGATSWQCKSDADISFTWG
                     NQPDEIVLHERNGKSSARREGGHTIKLDQLCNYSNTHWRYRCNLYLLSYELTSMRTTRY
                     QWKKRGLNVKIAPVENCPSEISPQTPEMLESPKLHAEGRGLGGQNKSVKNENPQEVDTV
                     SNAFAPGGSEWAKTKAMLLAKQAERRQKRWDSLNSI"
     misc_feature    1152..1157
                     /label=MfeI
                     /note="MfeI"
     regulatory      complement(1893..2554)
                     /note="PrepA; 662 nts upstream of repA-pFDA; derived from 
                     pPL2.7; Fremyella diplosiphon PCC7601"
                     /regulatory_class="promoter"
     misc_feature    2417..2422
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    2555..2560
                     /label=MfeI
                     /note="MfeI"
     misc_feature    2561..2581
                     /note="C3G3; GC-adaptor for seamless assembly, flanking 
                     cyanobacterial replicons; synthetic"
     misc_feature    2579..2584
                     /label=ZraI
                     /note="ZraI"
     misc_feature    2603..2608
                     /label=XbaI
                     /note="XbaI"
     primer_bind     complement(2645..2661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2669..2685)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2693..2723)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2738..2759)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3047..3635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3809..4666)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4667..4771)
                     /label=AmpR promoter
     misc_feature    4797..4802
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgaggacgtc gggggccccc
      421 ggggggattc tagattccac tgctactagt ggcgctagtg gcgctcacag gattaaataa
      481 gggcataaaa atacctttgt tttaagtaaa aatcacttcg cccagcataa tctcatcgct
      541 tcaaaaaagc gatcgcctgt gaataaatag ctgaaatgct tacatcatat agagttcaga
      601 ctatcccatc gtttttgcct tctctcggct tgcttggcca gtagcatagc cttagttttt
      661 gcccactcag aaccccctgg agcgaacgca ttgcttactg tgtctacttc ctggggattc
      721 tcatttttga cactcttatt ctgacccccc aaaccacgcc cttcggcgtg aagtttgggg
      781 gattcaagca tttcgggggt ttggggggaa atctcacttg ggcagttttc cacaggtgca
      841 attttgacgt ttaacccgcg tttcttccac tgatagcgag ttgtcctcat cgaggtcaac
      901 tcgtagctga gaaggtacaa attacagcga tagcgccaat gtgtattgct gtagttgcac
      961 agctgatcca gtttgattgt gtgccccccc tcacgcctag ctgatgattt cccgtttctc
     1021 tcatggagta caatttcatc gggctgattg ccccaggtga agctgatgtc agcatcactc
     1081 ttgcactgcc agctagtagc cccctgcttg attctgtgag cgatttcccg gtattcccgg
     1141 cgggcttgca tcaattgcct gtgttttacc tccaaacgct caacgtgtct ttggactgtg
     1201 cgaactgaaa caccacagct ggccggctgg gagtttagct cattgctgat ccgcttctga
     1261 gaaaccccaa aggggatgaa cgagcggcca acaaaaatcc gcttctcgcc aacgtgtaac
     1321 actccagttg ttcccccact agtcagcttc ggtgaggttt ggggtgttag cacgtcggca
     1381 gtagccggaa tctcaaaaca tttacgctca agcgggttaa gttgattttt tgccgcgtat
     1441 cgtgagcgct cttgccaatc ttgaattgtc atcgctgtag cgacagtgcg acgaccatta
     1501 ttttttaaaa tttcttctag tgggatgttg ttggccactg tgccccaatt ttttattctt
     1561 gcttttttgc aagcgcgcca caggctaccc aggctaattt ttaaagtgtt ccccgcccag
     1621 gagtaacaac gaaatagccc caaatcctta ccatccttga gccaacggta aattgttgat
     1681 tttttttcct gcaagagttc cagattttga ggagacaaaa ctacataacc acgaccactc
     1741 agatccaacg ctcgacacaa ataccacaat ttagaagccg gagtgagcag gagaattgaa
     1801 gcgtgtaatc ttgtttgtag tacaaaattc tccccctctg tagcggtgga ttgtgctata
     1861 atattggaat gtggaaatgt agtatgtttc acaaatcgcc ccttttatag cgacttgctg
     1921 acacatcacc ccaagctttc caacagcttt atattttcac gctcaatatt tgttaatttt
     1981 tagttagaga tttatttttg ttggcttgtt ggcgcgtctg ggttttttga tgtgtctgca
     2041 agtcattttt ttatgtgtcg cttcatgtca ggttgtcatg gtgttgtagc gtttattgca
     2101 tttattgctt gcaatttcct cctaattgaa ttaaggaatt aatggggcga tttaaaaatc
     2161 gccctggtgt ctacattcca gtagtttgcg gctactggga aataccgctc tctagcggca
     2221 cttcaaattt atgataagcg aaaatgaagg gttttcgctt gatagtaccg tgtcggcttt
     2281 tttacaaatt cggaaagccc ttggactgtc acgtagccag tttgggctgg aactgggtat
     2341 tgatgccgat acaataggga aatgggagaa ttcaaaagtt aacgtagcgc taactatcgc
     2401 ccaaatcaag cgattagata tctatttaaa agaattaggg ttatcctggc aggacttgcc
     2461 agatcatgtt ggcaacccag caatcgcccc gccagcatac aagcgcatca gaaaaaaaat
     2521 taaataacca tgtatatacg tggctagtca ttttcaattg atccccgggc ccgggcccga
     2581 cgtcctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat
     2641 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2701 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2761 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2821 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2881 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2941 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3001 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3061 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3121 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3181 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3241 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3301 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3361 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3421 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3481 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3541 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3601 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3661 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3721 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3781 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3841 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3901 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3961 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4021 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4081 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4141 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4201 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4261 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4321 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4381 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4441 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4501 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4561 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4621 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4681 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4741 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4801 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4861 ttcgtc
//
